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Enzymes as Sensors, Volume 589 - 1st Edition

Subject ISBN Author Publisher Number of Pages Title Year Price
Biotechnology 9780128054062 Richard Thompson, Carol A. Fierke Elsevier 530 Enzymes as Sensors, Volume 589 - 1st Edition 2017 $ 199.00
Author: Richard Thompson, Carol A. Fierke
Description: Enzymes as Sensors, Volume 589, the latest release in the Methods in Enzymology series, covers a variety of topics, including advances in genetically coded fluorescent sensors, enzymes as sensors, and bioapplications of electrochemical sensors and biosensors. Users will find a comprehensive discussion of timely topics that presents a micro-level delivery of specific content related to the study of enzymes in sensors. New to this edition are highly specialized chapters on integrated strategies for gaining a systems level view of dynamic signaling networks, sensitive protein detection and quantification in paper-based microfluidics for point-of-care, and microneedle enzyme sensor arrays for continuous in vivo monitoring. This state-of-the-art series is ideal for those interested in the latest information on enzymology, with this edition focusing on sensors and their role in enzymes.
Table of Content: Chapter One: Recent Advances in Development of Genetically Encoded Fluorescent Sensors • Abstract • 1 Introduction • 2 Fluorescent Proteins • 3 Sensor Platforms • 4 Types of Sensors • 5 Conclusion Chapter Two: Engineering Rugged Field Assays to Detect Hazardous Chemicals Using Spore-Based Bacterial Biosensors • Abstract • 1 Introduction • 2 Whole Cell-Based Biosensors • 3 Design, Creation, and Use of WCBs • 4 The Development of Spore-Based Bacterial Biosensors • 5 Challenges, Innovations, and Future Directions • 6 Conclusions • Acknowledgments Chapter Three: Engineering BRET-Sensor Proteins • Abstract • 1 Introduction • 2 LUCIDs and LUMABS: BRET-Sensors for Diagnostic Applications • 3 BRET/FRET-Sensors for Intracellular Measurements • 4 A Bright Future for BRET-Sensors • Acknowledgments Chapter Four: Enzymes as Sensors • Abstract • 1 Introduction • 2 Optical Biosensors • 3 Label-Free Detection and Fluorescence-Based Detection • 4 Why Choose Biomolecules for Sensing? • 5 Enzymes as MREs • 6 Thermophilic Enzymes • 7 Biological Engineering • 8 Conclusions • Acknowledgments Chapter Five: Integrated Strategies to Gain a Systems-Level View of Dynamic Signaling Networks • Abstract • 1 Introduction • 2 Strategies to Characterize Global Changes in the Phosphorylation Status of Cellular Proteins • 3 Strategies to Track Kinase and Phosphatase Activity Profiles in Living Cells • 4 Conclusions • Acknowledgments Chapter Six: Probing Cdc42 Polarization Dynamics in Budding Yeast Using a Biosensor • Abstract • 1 Introduction • 2 Generation of a Biosensor of Active Cdc42 • 3 Detection and Quantification of Cdc42 Activation In Vivo • 4 Application of the Cdc42 Biosensor • 5 Concluding Remarks • Acknowledgments Chapter Seven: Novel Fluorescence-Based Biosensors Incorporating Unnatural Amino Acids • Abstract • 1 Introduction • 2 Site-Specific Incorporation of Unnatural Amino Acids Into Proteins in Live Cells • 3 An Unnatural Fluorescent Protein Biosensor for Hydrogen Peroxide • 4 Concluding Remarks • Acknowledgments Chapter Eight: Folding- and Dynamics-Based Electrochemical DNA Sensors • Abstract • 1 Introduction • 2 “Signal-Off” E-DNA Sensors • 3 “Signal-On” E-DNA Sensors • 4 Alternate Electrode Substrates • 5 Alternate Sensor Fabrication Approaches • 6 Conclusion and Outlook • Acknowledgment Chapter Nine: Construction of Protein-Based Biosensors Using Ligand-Directed Chemistry for Detecting Analyte Binding • Abstract • 1 Introduction • 2 Expression of GABAAR Consisting of α/β/γ Subunits in HEK293T Cells • 3 Design and Synthesis of Labeling Reagents for GABAAR • 4 Chemical Labeling of GABAAR Using LDAI Reagent • 5 Construction of Fluorescent Biosensors Using Labeled GABAARs • 6 Quantitative Analysis of Ligand Affinity Using BFQR-Based Biosensors • 7 Summary and Future Directions • Acknowledgments Chapter Ten: Measuring and Imaging Metal Ions With Fluorescence-Based Biosensors: Speciation, Selectivity, Kinetics, and Other Issues • Abstract • 1 Introduction • 2 Speciation of Metal Ions of Biological Interest • 3 Metal Ion Buffers and Their Formulation • 4 Selectivity of Metal Ion Binding to Indicators and Its Assessment • 5 Advantages of Biomolecules for Metal Ion Sensing • 6 Kinetics and Mechanism of Metal Ion Binding • 7 Distribution of Metal Ions Throughout the Cell and Organism • 8 Conclusion Chapter Eleven: Bioapplications of Electrochemical Sensors and Biosensors • Abstract • 1 Introduction • 2 Electrochemical Sensing in Biological Systems • 3 Recent Advancements in Electrode Designs for Biological Monitoring • 4 Conclusions and Future Trends • Acknowledgments Chapter Twelve: A Highly Sensitive Biosensor for ATP Using a Chimeric Firefly Luciferase • Abstract • 1 Introduction • 2 ATP Detection Protocol • 3 Conclusions • Acknowledgments Chapter Thirteen: Highly Modular Bioluminescent Sensors for Small Molecules and Proteins • Abstract • 1 Introduction • 2 Sensing Small Molecules • 3 Sensing Proteins • 4 Toward Application in POC Settings • 5 Conclusions Chapter Fourteen: Sensitive Protein Detection and Quantification in Paper-Based Microfluidics for the Point of Care • Abstract • 1 Introduction • 2 Paper-Based Devices and Sandwich Lateral Flow Assays • 3 Assay Analysis Framework • 4 Binding Kinetics in Porous Networks • 5 Mechanisms for Protein Binding • 6 Optical Methods in Paper • 7 Sensitivity Enhancements (LOD) • 8 Summary and Next Steps Chapter Fifteen: Microneedle Enzyme Sensor Arrays for Continuous In Vivo Monitoring • Abstract • 1 Introduction • 2 Approaches to the Fabrication of Microneedles for Sensing • 3 Functionalization of Microneedles for Sensing • 4 Methods • Acknowledgments Chapter Sixteen: Visualization of the Genomic Loci That Are Bound by Specific Multiprotein Complexes by Bimolecular Fluorescence Complementation Analysis on Drosophila Polytene Chromosomes • Abstract • 1 Introduction • 2 Design of Fusion Proteins for BiFC Analysis • 3 Construction and Testing of Plasmid Vectors for BiFC Analysis • 4 Production of Drosophila Lines That Express BiFC Fusion Proteins • 5 Comparison of the Levels of BiFC Fusion Protein Expression • 6 Preparation of Drosophila Larvae for Salivary Gland Isolation • 7 Preparation of Polytene Chromosome Spreads • 8 Staining and Imaging of Polytene Chromosomes • 9 Interpretation of BiFC Complex Binding on Polytene Chromosomes • 10 Strategies to Limit the Artifacts Caused by the Differences Between Fusion Proteins and Endogenous Counterparts • 11 Effects of BiFC Complex Formation on the Specificity of Chromatin Binding • 12 Materials and Solutions

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