||Number of Pages
||Reactive Species Detection in Biology - 1st Edition
Author: Frederick Villamena
Description: Reactive Species Detection in Biology: From Fluorescence to Electron Paramagnetic Resonance Spectroscopy discusses the reactive oxygen species that have been implicated in the pathogenesis of various diseases, presenting theories, chemistries, methodologies, and various applications for the detection of reactive species in biological systems, both in-vitro and in-vivo. Techniques covered include fluorescence, high performance chromatography, mass spectrometry, immunochemistry, and electron paramagnetic resonance spectroscopy. Probe design and development are also reviewed in order to advance new approaches in radical detection through synthesis, computations, or experimental applications.
Table of Content: Dedication Preface Chapter 1. Introduction References Chapter 2. Chemistry of Reactive Species Abstract 2.1 Introduction 2.2 Redox Chemistry 2.3 Properties of Reactive Species References Chapter 3. Reactive Species in Biological Systems Abstract 3.1 Introduction 3.2 Extracellular Milieu 3.3 Membrane-Bound Enzymes 3.4 Cytosolic Enzymes 3.5 Organelle Enzymes References Chapter 4. Fluorescence Technique Abstract 4.1 Introduction 4.2 Fluorescence Spectroscopy and Microscopy 4.3 Chemistry of Redox Detection by Fluorescence 4.4 Classification of Fluorescent RS Probes by Specificity 4.5 Considerations in Fluorescence Probe Application References Chapter 5. EPR Spin Trapping Abstract 5.1 Introduction 5.2 Electron Paramagnetic Resonance Spectroscopy 5.3 Chemistry of Spin Trapping 5.4 Classification of Spin Traps 5.5 Kinetics and Thermodynamics of Spin Trapping and ph effect 5.6 Kinetics and Thermodynamics of Adduct Decay 5.7 Biostability and Cytotoxicity of Spin Traps 5.8 Synthesis of Spin Traps 5.9 Interpretation of EPR Spectra 5.10 Applications of Spin Trapping References Chapter 6. UV–Vis Absorption and Chemiluminescence Techniques Abstract 6.1 Introduction 6.2 Superoxide Radical Probes 6.3 Hydroxyl Radical Probes 6.4 Antioxidant Capacity Assays 6.5 Nitric Oxide and Metabolites Probes 6.6 Thiols Probes 6.7 Peroxides Probes 6.8 Chemiluminescence References Chapter 7. Electrochemical, Mass Spectroscopic, Immunochemical, and Nuclear Magnetic Resonance Techniques Abstract 7.1 Introduction 7.2 Electrochemical Techniques 7.3 Mass Spectroscopy 7.4 Immunochemical Technique 7.5 Nuclear Magnetic Resonance Spectroscopy and Imaging References Index